Skip to main content
. 2014 Jun 11;307(3):C278–C287. doi: 10.1152/ajpcell.00391.2013

Fig. 5.

Fig. 5.

SMAD3 augments FoxO3 binding within the proximal promoter region of MuRF-1. HEK293a cells were transfected with plasmid DNA encoding the 0.25-kb MuRF-1 reporter (wild-type or SBE-mut), SMAD3 and/or FoxO3, and chromatin immunoprecipitation was performed. A and B: SMAD3 binding to the wild-type and SBE-mut reporters. C and D: FoxO3 binding to the wild-type and SBE-mut reporters. E and F: IgG (control antibody) binding to the wild-type and SBE-mut reporters. Note the differential y-axes scale bars between A, C, E and B, D, F. Results from this experiment were confirmed on two independent occasions.