Phylogenetic analysis of amino acid sequences of cloned rainbow trout acid-sensing ion channels ASIC1 and ASIC4 and ASICs from various fish species. Phylogenetic tree was constructed using the maximum-likelihood method, and numbers indicate bootstrap values for 700 resamplings. GenBank or Ensembl accession numbers are as follows: human ASIC2, NP_001085.2; Western clawed frog ASIC2, XP_002933086.2; Japanese pufferfish ASIC2, XP_003964871.1; zebrafish ASIC2, NP_999953.1; three-spined stickleback ASIC2, ENSGACG00000009558; Atlantic cod ASIC2, ENSGMOT00000019145; spotted green pufferfish ASIC2, ENSTNIG00000014943; Southern platyfish ASIC2, ENSXMAG00000008283, oyster toadfish ASIC2, AAP45633.1; coelacanth ASIC2, ENSLACG00000007191; rat ASIC2, NP_037024.2; Southern platyfish ASIC1c, ENSXMAG00000018841; African clawed frog ASIC1.1, AEC48665.1; African clawed frog ASIC1.2, AEC48667.1; brown rat ASIC1, NP_077068.1; human ASIC1, NP_064423.2; three-spined stickleback ASIC1a, ENSGACG00000003075; Japanese pufferfish ASIC1, XP_003963520.1; Southern platyfish ASIC1a, XP_005801801.1; Atlantic cod ASIC1a, ENSGMOG00000009659; zebrafish ASIC1.2, NP_999955.1; rainbow trout ASIC1, KF964646; Atlantic salmon ASIC1, NP_001133456.1; oyster toadfish ASIC1, Q7T1N4.1; elephant shark ASIC1, AEC48668.1; three-spined stickleback AIC1c, ENSGACG00000017974; Atlantic cod ASIC1c, ENSGMOG00000003105; zebrafish ASIC1.3, NP_999954.1; river lamprey ASIC1, AAY28983.1; sea lamprey ASIC1b, ENSPMAG00000003813; dogfish shark ASIC1, AAY28985.1; zebrafish ASIC1.1, NP_999956.1; human ASIC4, NP_878267.2; rat ASIC4, NP_071570.2; African clawed frog ASIC4, AEC48666.1; Japanese pufferfish ASIC4, XP_003962127.1; spotted green pufferfish ASIC4b, ENSTNIG00000010994; Nile tilapia ASIC4, XP_003445446.1; Southern platyfish ASIC4a, ENSXMAG00000003624; three-spined stickleback ASIC4a, ENSGACG00000007052; Atlantic cod ASIC4a, ENSGMOG00000012740; rainbow trout ASIC4, KF964645; zebrafish ASIC4.1, NP_999952.1; zebrafish ASIC4.2, NP_999951.1.