Table 1.

Primer sets for cloning and tissue distribution of ASICs

Gene	Primer Sequence
	Cloning
ASIC1
5′	5′-TCACCCAGCAACCCTGCGAACTCGT-3′
3′	5′-ACACCCTGCAACATGACGCGCT-3′
ASIC4
5′	5′-GATGTCCCGTCCGGTTGAAGATGTC-3′
Forward	5′-CCCAGTAACATCAAGTGTGTCG-3′
Reverse	5′-YYARCANGCRAARTCYTC-3′
	RT-PCR
ASIC1
Forward	5′-AAGTCCACTCCCATAGA-3′
Reverse	5′-CAGCCAGGTTATTCCTT-3′
ASIC4
Forward	5′-CTTTCGTTTCTCTGCTCTCACC-3′
Reverse	5′-CAAGACCAGGAAGTTGTCTCTG-3′
GAPDH
Forward	5′-AAGGGTGAGGTGAGCATGGA-3′
Reverse	5′-GCTTTACCCCATGGGATCTCAT-3′

ASIC, acid-sensing ion channel; 5′, 5′-rapid amplification of cDNA ends (RACE) primer; 3′, 3′−RACE primer; Y = C + T; R = A + G; N = A + G + T + C.