Fig. 5.

Analysis of CPI-17 (PPP1R14A) and PHI-1 (PPP1R14B) noncoding sequence for potential transcriptional regulatory activity. A: protein phosphatase 1 regulatory subunit 14A (PPP1R14A). Portions of noncoding sequence in introns 1 and 3 (red boxes 1 and 2) are well conserved between human and mouse and have other indicators suggestive of transcriptional enhancer activity (H3 modification, DNase hypersensitivity, and TF ChIP). The transcription factor binding sites (TFBS) predicted in each of these blocks of sequence are shown. The TFBS in black are predicted using the rVista “optimized for function” approach to reduce false positives (see methods). The TFBS in gray have a singular cutoff of 0.85 similarity to the TRANSFAC positional weighted matrix (PWM) and are of interest to muscle gene regulation. B: PPP1R14B. noncoding sequence is well conserved immediately upstream of the TSS and in intron 1 (red boxes 1 and 2) and have other features suggestive of transcriptional promoter and enhancer activity, respectively. The TFBS predicted in each of these blocks of sequence are shown. The full compilation of TFBS for the two cutoffs can be found in Supplemental Tables S3 and S4.