Fig. 4.
The EPEC locus of enterocyte effacement (LEE) is necessary and sufficient to promote EGFR degradation. C2BBE cells were treated with medium alone or infected with EPEC, DH5α harboring the cosmid vector pCVD551 (DH5α/vector) or DH5α harboring the LEE-encoding pCVD462 (DH5α/pLEE) at MOI = 100. Total protein extracts isolated at specific time points were immunoblotted for EGFR and actin. Blots shown are representative of 3 similar experiments. Densitometric analysis was performed to determine EGFR abundance relative to actin and normalized to levels in control uninfected cells. P values for specific conditions compared were assessed by ANOVA with Bonferroni post hoc test; *P < 0.01. Error bars represent standard error.