Fig. 2.

Protein levels of zinc-binding proteins in mouse livers. Mice were chronically fed an ethanol or control diet for 2, 4, or 8 wk. The immunoblot bands of peroxisome proliferator-activated receptor-α (PPAR-α; A), hepatocyte nuclear factor 4α (HNF-4α; B), and superoxide dismutase 1 (SOD1; C) were quantified by densitometry. The ratio to α-tubulin was calculated by setting the value of 2 wk of control as 1. The activity of SOD1 (D) was determined by SOD1 assay kit. Results are expressed as means ± SD (n = 4). Significant differences (*P < 0.05) between groups are determined by Student's t-test.