Fig. 7.

CPS1 is detected in sera of human patients with acute liver failure (ALF). The presence of CPS1 in serum of human patients with different forms of ALF was tested by using serum collected from day 1 up to day 6 of hospitalization. A: analysis of CPS1 in serial serum samples collected from a patient with APAP. Sera were separated on duplicate gels by SDS-PAGE, with 1 gel stained by Coomassie blue to visualize the immunoglobulin heavy (H) and light (L) chains. The second gel was transferred to a membrane for subsequent blotting using anti-CPS1 antibody. ALT values from the same serum samples are also included. B and C: serial serum samples from a patient with ischemic hepatitis and with Wilson disease, respectively, were analyzed as in A.