Fig. 5.

A: images representing intestinal cross sections stained with the Apoptag in situ apoptosis detection kit and DAPI (4′,6-diamidino-2-phenylindole) (blue) and viewed under a microscope for apoptotic cells. The numbers of apoptotic cells per high-power field (HPF) were measured in 10 villi in 5–6 HPFs on each slide. B: graphical representation of the quantification of cells positive for apoptosis using the method stated in A. *P < 0.05, n = 3 in each group. C: quantitative PCR comparing relative mRNA expression of the BAX gene between controls and CKO. *P < 0.05, n = 4 in each group. D: representative Western blot of intestinal homogenates comparing levels of proapoptotic proteins BAX, AIF, and caspase-3 between CKO and controls, along with loading control GAPDH.