Fig. 10.

Differences in Th1, Th2, and Th17 and TGFβ receptors in whole lung do not explain the differential ability of γHV-68, but not H1N1, to exacerbate ECM deposition post-bleomycin. Mice were treated with bleomycin on day 0 and infected with saline, γHV-68, or H1N1 on day 14. On day 21, lungs were harvested and whole lung homogenates were analyzed by ELISA for IFNγ (A), IL-13 (B), and IL-17 (C); n = 5 for each group. In D and E, whole lung RNA was prepared and analyzed for expression of TGFβRI and II by real-time RT-PCR, n = 3–5 for each group; all representative of 2 experiments. *P < 0.05.