Fig. 12.

Alveolar epithelial cells (AECs) from bleomycin and γHV-68-treated mice show increased sensitivity to TGFβ signaling, and increased evidence of leukotriene synthesis and apoptosis post-γHV-68 infection. Mice were treated with bleomycin on day 0. On day 14, some mice were infected with saline, H1N1, or γHV-68. Primary AECs were isolated from the lungs of all groups of mice on day 21. Total RNA was isolated and by real time RT-PCR we measured the expression of TGFβ receptor I/II (A, B), 5-lipoxygenase (5-LO; C), and 5-LO activating protein (FLAP; D). Phosphorylated SMAD3, total SMAD3, cleaved PARP, total PARP, and beta actin were detected by Western blotting (E). Data in all panels represent n = 3/group. ****P < 0.0001.