Fig. 8.

Effects of compound 13 and bradykinin on Ca²⁺- activated chloride channel activity [spontaneous transient inward current (STICs)] from cultured human airway smooth muscle cells. A, a: representative tracing of Ca²⁺-activated chloride channel current (STICs) in response to 10 μM compound 13 in human airway smooth muscle cells. b: Representative tracing of STICs in response to 10 μM compound 13 and 30 μM bradykinin in human airway smooth muscle cells. B, a: summary of effects of compound 13 on STICs under basal conditions. Compound 13 decreased the basal amplitude (control 1 ± 0.26, compound 13 0.44 ± 0.09) and frequency of STICs (control 1 ± 0.29, compound 13 0.4 ± 0.1) (n = 9). b: Bradykinin increased STICs and compound 13 decreased the amplitude (control 1 ± 0.21 bradykinin 1.79 ± 0.11; bradykinin plus compound 13 0.46 ± 0.13) and frequency (control 1 ± 0.43, bradykinin 2.03 ± 0.85, bradykinin plus compound 13 0.4 ± 0.14) of these bradykinin-induced STICs; n = 5. *P < 0.05, **P < 0.01 all compared with control groups. Values expressed as fold change with control normalized to 1.