Fig. 5.

Lungs from hyperoxia-exposed neonatal mice show significantly lower expression of PDGFR-α and PDGFR-β. Two- to 3-day-old wild-type C57BL/6J mice were exposed to air or 75% oxygen for 14 days. Total lung mRNA and protein lysates were harvested. Lung mRNA encoding PDGFR-α (A) and PDGFR-β (B) was decreased after 14-day hyperoxic exposure (*different from same-day control, P < 0.05, unpaired t-test). C: immunoblotting analysis shows reduced protein expression of PDGFR-α (molecular weight, 170 kDa) and PDGFR-β (molecular weight, 190 kDa) in lungs from hyperoxia-exposed mice. Results are representative of 3 independent experiments.