Fig. 2.

mDia2 shuttles between the nucleus and cytoplasm. A: localization of green fluorescent protein (GFP) or GFP-tagged FHOD1, mDia1, or mDia2 in 10T1/2 cells in the absence and presence of leptomycin B (LMB). Scale bar = 20 μm. B: nuclear and cytoplasmic lysates were prepared from untreated 10T1/2 cells by differential centrifugation and were subjected to Western analysis using the indicated antibodies. Note that vinculin and histone 3 serve as controls for the cytoplasmic and nuclear fractions, respectively. C: control and leptomycin B-treated 10T1/2 cells were fixed and stained with anti-mDia2 antibody. Scale bar = 20 μm.