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. 2014 Jun 6;307(3):H379–H390. doi: 10.1152/ajpheart.01002.2013

Fig. 9.

Fig. 9.

Nuclear-targeted leukemia-associated Rho guanine nucleotide exchange factor (GEF) (LARG) and RhoA-stimulated MRTF activity. A: 10T1/2 cells were transfected with the GFP-tagged LARG variants shown. After 24 h, LARG localization was scored in at least 100 cells from 3 separate experiments. C-Ter, COOH-terminal. B: 10T1/2 cells were transfected with the indicated promoter-luciferase construct along with the indicated LARG nuclear localization variant. *P < 0.05 vs. empty vector. **P < 0.05 vs. Wt. C: quantification of GFP-MRTF-B localization in 10T1/2 cells coexpressing Wt or ΔC-ter LARG. At least 100 cells were counted under each condition from 3 independent experiments. *Nuclear localization P < 0.05 vs. empty vector. D: 10T1/2 cells were transfected with SM22-luciferase, MRTF-B, and either a cytoplasmic or nuclear-targeted variant of constitutively active L63RhoA. *P < 0.05 vs. empty vector. **P < 0.05 vs. L63RhoA. E: phalloidin staining of 10T1/2 cells expressing the cytoplasmic and nuclear-targeted variants of L63RhoA.