FIG. 3.
Complementation of the growth defect of B. subtilis DB3200 by the N-terminally truncated σA in vivo. The plasmids pCF0, pCF100, and pCF104 (see Materials and Methods) were introduced separately into B. subtilis DB3200. The growth curve of each strain of B. subtilis in minimal medium was then measured. ○ and □ indicate the growth of B. subtilis DB3200 in the presence and absence of IPTG, respectively. •, ▴, and ▪ represent the growth of B. subtilis DB3200/pCF0, DB3200/pCF100, and DB3200/pCF104, respectively, in the absence of IPTG. A550, absorbance at 550 nm.