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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Nat Biotechnol. 2014 May;32(5):453–461. doi: 10.1038/nbt.2890

Figure 7. Genomic features of circular RNA.

Figure 7

Circular RNAs are generated from exons that are longer than average, and are flanked by long introns containing inverted ALU repeats, as exemplified by the HIPK3 gene, shown with CircleSeq data set35. In the HIPK3 gene, ribosomal RNA depleted sequencing (green) resulted in substantially more coverage of a single exon, which also demonstrated a large number of backsplice sequences. These sequences are also demonstrated to be true circles by their enrichment by RNAse R (brown), while all other exons are degraded. This circularized exon is the longest of the gene, is surrounded by the longest introns of the gene, and is flanked by ALU elements in a complementary orientation