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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Arch Biochem Biophys. 2014 May 9;554:11–21. doi: 10.1016/j.abb.2014.04.017

Table 1. Myofilament protein phosphorylation before and after cTn exchange.

Values are means ± SEM of the ProQ/SYPRO intensity ratio. Shown is myofilament protein phosphorylation determined with ProQ-diamond staining before and after exchange in three IDCM samples. Troponin exchange did not affect myofilament protein phosphorylation (by one-way ANOVA comparing before exchange to the exchanged groups). cMyBP-C, cardiac myosin-binding protein-C; MLC2, myosin light chain 2; A, alanine substitution; D, aspartic acid substitution; Wt, wild-type.

cMyBP-C Desmin MLC2
Before exchange 0.43±0.01 0.58±0.15 0.40±0.10
Wt 0.41±0.03 0.59±0.07 0.34±0.04
42D/44D 0.41±0.05 0.63±0.20 0.37±0.10
42A/44A 0.41±0.04 0.62±0.20 0.47±0.18