TABLE 2.

CAT-specific activities in wild-type and CcpA-deficient strains of S. gordonii/p_arcA-cat and wild-type and Flp-deficient strains of S. gordonii/p_arcA-cat under aerobic and anaerobic growth conditions

S. gordonii strain	CAT-specific activity under the indicated conditionsa
	With galactoseb	With glucoseb	Anaerobicc	Aerobicc
WTd	1,024 ± 17	60 ± 6	794 ± 28	159 ± 21
CcpA deficient	907 ± 15	882 ± 35
Flp deficient			70 ± 14	37 ± 6

^aThe values presented are the means and standard deviations of three independent experiments and are expressed as nanomoles of chloramphenicol acetylated per minute per milligram of total protein. All CAT assays were done in triplicate.

^bCells were grown in 5% CO₂ and 95% air and TY medium with the carbohydrate as indicated.

^cAll cultures were grown in TY medium-10 mM galactose-50 mM arginine under anaerobic conditions in a GasPak jar or under aeration conditions with shaking at 300 rpm (see text for details).

^dWT, wild type.