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. 2014 Jul-Aug;30(4):850–853.

Table-I.

Cycling conditions of RT-PCR in BCR-ABL

Segment Number of cycles Temperature Duration
Initial duration 01 940C 15 mins
Denaturation 37 940C 0.5 min
Annealing 37 600C 1.5 mins
Extension 37 720C 1.5 mins
Final extension 01 720C 10 mins