Table-I.
Segment | Number of cycles | Temperature | Duration |
---|---|---|---|
Initial duration | 01 | 940C | 15 mins |
Denaturation | 37 | 940C | 0.5 min |
Annealing | 37 | 600C | 1.5 mins |
Extension | 37 | 720C | 1.5 mins |
Final extension | 01 | 720C | 10 mins |
Segment | Number of cycles | Temperature | Duration |
---|---|---|---|
Initial duration | 01 | 940C | 15 mins |
Denaturation | 37 | 940C | 0.5 min |
Annealing | 37 | 600C | 1.5 mins |
Extension | 37 | 720C | 1.5 mins |
Final extension | 01 | 720C | 10 mins |