FIG. 6.

GldI is a lipoprotein. (A) Cells of F. johnsoniae were labeled with either [³H]glutamate (to label nearly all proteins) or [³H]palmitate (to label lipoproteins). Proteins were separated by SDS-PAGE and detected by autoradiography. Lane 1, wild-type cells labeled with [³H]glutamate; lane 2, wild-type cells labeled with [³H]palmitate; lane 3, cells of the gldI mutant UW102-41 labeled with [³H]palmitate; lane 4, cells of UW102-41 complemented with pMM291 (which carries gldI) labeled with [³H]palmitate. (B) Radiolabeling of GldB-His and GldI-His. Cells of F. johnsoniae were labeled with [³H]palmitate or [³H]glutamate. Proteins were isolated by precipitation with Ni-NTA His-Bind resin, separated by SDS-PAGE, and detected by autoradiography. Lane 1, cells expressing GldB-His from pTB39, labeled with [³H]palmitate; lane 2, cells expressing GldB-His from pTB39, labeled with [³H]glutamate; lane 3, cells expressing GldI-His from pTB45, labeled with [³H]palmitate; lane 4, cells expressing GldI-His from pTB45, labeled with [³H]glutamate.