TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Known genotypea | Reference or source |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 flbB5301 deoC1 relA1 rbsR rpsL150 ptsF25 | 3 |
| BL21 | F−ompT hsd SB(rB− mB−) gal dcm | 37 |
| IBPC5321 | thi-1 argG6 argE3 his-4 mlt-1 xyl-5 rpsL ΔlacX74 mlc-1 | 28 |
| IBPC590 | IBPC5321 ΔnagEBADC::Tetr | 28 |
| IBPC531 | IBPC5321 nagA::Cmr | 28 |
| IBPC546 | IBPC5321 nagB::Kmr | 28 |
| IBPC542 | IBPC5321 nagE::Kmr | 28 |
| KM540 | MC4100 treA::Spcrpgi | 13 |
| LR-2-167 | argG6 galT his-1 man1 metB phoA rpsL thi-1 nagE ptsM | 41 |
| LR-2-168 | LR-2-167 ptsG | 40 |
| LM1 | LR-2-167 crr | Josef Lengeler, Osnabrück |
| PPA69 | HfrK16 galR thi glk::Cmr ΔptsHIcrr | Lab stock |
| ET185 | MC4100 φP[(malT-lacZ)(λplacMu50)] glpK::Cmr Δcrr::Kmr | Tanja Eppler, Konstanz |
| ET25 | MC4100 Δcya crp* ilv+ | Tanja Eppler, Konstanz |
| JJ3 | ET25 Δcrr::Kmr | This study |
| CM1 | JJ3 ΔtreA | This study |
| DY330 | W3110 ΔlacU169 gal-490 (λ cI857 Δcro/broA) | 47 |
| CM99 | DY330 yfeV::Kmr | This study |
| CM100 | MC4100 yfeV::Kmr | This study |
| CM101 | KM553 yfeV::Kmr (ptsHIcrr)+ | This study |
| CM102 | KM553 yfeV::Kmr ΔptsHIcrr | This study |
| CM103 | MC4100 ΔyfeV | This study |
| Plasmids | ||
| pKD4 | Apr Kmr plasmid, PCR template for homologous gene displacement | 6 |
| pCP20 | Apr Cmr plasmid that shows temperature-sensitive replication and thermal induction of FLP recombinase synthesis | 6 |
| pCS19 | Expression vector based on pQE31 (Qiagen) Apr, additionally carrying the constitutively expressed lac repressor lacIq | 36 |
| pCS19YfeV | yfeV cloned into pCS19, expression vector for IPTG-inducible MurP expression | This study |
| pCS19YfeV His6 | yfeV cloned into pCS19, expression vector for IPTG-inducible MurP His6 expression | This study |
a
Apr, ampicillin resistant; Cmr, chloramphenicol resistant; Kmr, kanamycin resistant; Spcr, spectinomycin resistant; Tetr, tetracycline resistant.