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. 2014 Jul 15;111(30):11199–11204. doi: 10.1073/pnas.1411859111

Fig. 1.

Fig. 1.

Cloning and characterization of SOL1. (A) Phenotypes of wild-type (ZH11), la1, and la1 sol1-1 plants at the adult stage. (Scale bar: 10 cm.) (B) Map-based cloning of SOL1. SOL1 was pinpointed in an 80-kb genomic region between molecular markers M8 and M10 and cosegregated with markers M9 and M11 on chromosome 6. Numbers under the markers indicate recombinants. The blue arrow indicates an R702Q amino acid substitution in sol1-1, and the green arrow indicates one base deletion at the first exon of LOC_Os06g06050 that results in a premature translation in sol1-2. The closed box indicates an ORF, and open boxes indicate UTRs. (C) Comparison of tiller angle in the wild-type (Shiokari), la1, and D3RNAi transgenic plants. (Scale bar: 10 cm.) (D) The gravitropic response of D3RNAi/la1 plants at the seedling stage after 24 h gravistimulation. Light-grown seedlings (3 d old) were transferred to darkness and reoriented by 90°. Error bars indicate SEM; n = 10. Means with different letters are significantly different (P < 0.01; ANOVA).