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. 2014 Jul 14;111(30):11055–11060. doi: 10.1073/pnas.1409314111

Fig. 2.

Fig. 2.

LC3 and SQSTM1 are reduced in G85R SOD1YFP motor neurons compared with WT SOD1YFP. Spinal cord slices were stained for autophagosome markers LC3 or SQSTM1 with the respective antibodies and Alexa Fluor 568 secondary antibody. The sections were treated with copper sulfate to remove lipofuscin auto-fluorescence then imaged in the red channel. (A) For LC3, the bar graphs show the fraction of cells of each genotype that had >15 vs. ≤15 puncta. (B) For SQSTM1, the bar graphs show the fraction of cells of each genotype that had >30 puncta, 15–30 puncta, or <15 puncta. Most WT cells had a relatively large number of LC3 and SQSTM1 puncta, whereas the majority of the mutant G85R cells had substantially fewer, indicating that autophagosomal proteins are reduced in G85R SOD1YFP motor neuron cell bodies. Data are presented as means and SD for the indicated number of mice.