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Test for the reversibility of the molten globule state for mutant A31P by CD (A) Melting curve for A31P produced in the absence of β-met. (B) Melting curve of a sample from the original protein after a 7-d storage at 4 °C. (C) Melting curve obtained after a 7-d storage at 4 °C of a sample from the original protein to which β-met has been added before storage. The CD signal is transformed into mean residue ellipticity.
