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. 2014 Jul 14;111(30):E3062–E3071. doi: 10.1073/pnas.1411370111

Fig. 2.

Fig. 2.

shRNA-mediated p21 knockdown in unrelated human cancer cell lines sensitizes them to telomerase inhibition-mediated apoptosis. Analysis of RKO (A–E) and ACHN (F–J) cells stably transduced with nonspecific control (NS) or two different p21 shRNAs. (A–E and F–J) Cells were treated with mismatch oligonucleotide or with imetelstat for 6 wk. (A and F) Colony formation monitored by crystal violet staining. (B and G) Cell viability was measured by trypan blue exclusion assay. Cell viability relative to mismatch oligonucleotide is plotted. (C and H) Telomerase activity as measured by the TRAP assay and plotted relative to the mismatch oligonucleotide. (D and I) Cleaved caspase 3 immunoblot to measure apoptosis. Actin was used as a loading control. (E and J) % Annexin V–FITC–positive cells under indicated treatment conditions. (K and L) Average tumor volumes for indicated cell lines for indicated conditions are shown. **P < 0.001; ***P < 0.0001.