TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| NovaBlue | Recipient for cloning experiments | Novagen |
| BL21(DE3)/pLysS | Used for overproduction of proteins from pET-based vectors | Novagen |
| Salmonella | ||
| JR501 | Used for converting E. coli plasmids to Salmonella compatibility | 28 |
| SJW1103 | Wild type | 33 |
| SJW170 | fliR null | 33 |
| MKM50 | flhB null strain | 6 |
| MKM65 | fliR null strain | This study |
| MKM58 | fliR flhB double-null strain | This study |
| Plasmids | ||
| pET19b | N-terminally His-tagged T7 expression vector | Novagen |
| pET19bFH | N-terminally His-FLAG-tagged T7 expression vector | 4 |
| pTrc99A-FF4 | Modified pTrc expression vector | 27 |
| pMM26 | pTrc99A-FF4/FlhB | 22 |
| pRMK3000 | pET19b/N-His-FliR | This study |
| pGFBN269A | pet19b/N-His-FlhBN269A | 6 |
| pJSV003 | pET19bFH/N-His-FLAG-FliR-FlhB | This study |
| pJSV004 | pTrc99A-FF4/FliR-FlhB | This study |
| pJSV013 | pET19bFH/N-His-FLAG-FliR-FlhBN269A | This study |
| pJSV014 | pTrc99A-FF4/FliR-FlhBN269A | This study |
| pJSV024 | pTrc99A-FF4/FliR + FlhB | This study |