Fig. 4.

A cas9 deletion mutant is rescued for virulence in mice lacking both ASC and TLR2. (A and B) WT or ASC/TLR2-deficient mice were inoculated s.c. with 10⁵ cfu of WT or the cas9 deletion strain. Forty-eight hours postinfection, the (A) spleen and (B) liver were harvested and plated to quantify bacterial levels (n = 5). (C and D) Groups of 15 (C) WT or (D) ASC/TLR2-deficient mice were inoculated s.c. with 10⁸ cfu of WT or cas9 deletion strains. Mice were monitored for survival over 15 d. Data are representative of at least two independent experiments in A and B; data are compiled from three independent experiments for C and D. **P ≤ 0.005.