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. 2014 Jul 17;111(30):11169–11174. doi: 10.1073/pnas.1404739111

Fig. 3.

Fig. 3.

Effects of cervid PrP substitutions on PMCA of CWD and SSBP/1. (A and C) PMCA using PrPC substrates from Tg mice. (A) Brain extracts from D10 deer or G/S96 deer 135 were used as seeds. (C) SSBP/1 adapted in Tg(DeerPrP) mice was used as seed. “+PMCA” and “–PMCA” indicate whether or not samples were subject to sonication. The first two lanes of the +PMCA panels are brain homogenates from uninfected Tg mice, untreated or treated with PK, respectively. All other samples were PK-treated. (B) Serial PMCA using PrPC from Tg(DeerPrP) or Tg(ElkPrP) mice seeded with sheep SSBP/1 prions. Numbers refer to rounds of serial PMCA. (Upper and Lower) The left four lanes contain undigested substrate; undigested SSBP/1; PK-treated SSBP/1; and unsonicated material from round 1. All other samples were PK-treated. Blots were probed with mAb 6H4.