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. 2014 Jul 14;111(30):11145–11150. doi: 10.1073/pnas.1404139111

Fig. 2.

Fig. 2.

Pla2g16 contributes to the metastatic potential of p53R172H/+ osteosarcomas. (A) shRNA knockdown of Pla2g16 in p53R172H/+ osteosarcoma cell lines H76 and H318-1 was determined by qRT-PCR (Upper) and Western blot analysis (Lower). Migration (B) and invasion (C) assays were performed using Pla2g16 knockdown cells for 16 h and 40 h, respectively. (D) Colony formation assays were performed for Pla2g16 knockdown cells. (E) Number of mice with or without metastasis after injection with control and Pla2g16 knockdown cells. (F) Overexpression of Pla2g16 in p53–/o osteosarcoma cells increased invasion. Pla, Pla2g16; Flag, Flag-tag; Vin, vinculin; Ctrl, shRNA of EGFP; shPla, shRNA of Pla2g16; vec, pBabe-puro; *P < 0.05 and ***P < 0.0005.