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. 2014 Jul 14;111(30):11145–11150. doi: 10.1073/pnas.1404139111

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Fig. 4. — Up-regulation of Pla2g16 by p53 mutants. (A) Western blots of H318-1 cells with and without p53R172H knockdown. (B) Western blots of p53^−/− MEFs and p53^–/o osteosarcoma cell line (#222) with addition of p53R172H. (C) Western blots for p53R175H overexpression in Saos2 cells with Pla2g16 shRNA knockdown. (D) shRNA knockdown of Pla2g16 in p53R175H expressing Saos2 cells inhibited colony formation. shp53, shRNA for p53; shpla, shRNA for Pla2g16; Vec, pBabe vector for overexpression of p53R175H. **P < 0.005, ***P < 0.0005.