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Complementation of FtsY depletion by various truncated FtsY mutants. (A) Schematic representation of the mutants. (B) The activity of the mutants was tested by monitoring the growth in arabinose-depleted 2YT medium of E. coli FJP10 transformed with the different FtsY constructs. Extracts (5 μg of protein) of noninduced E. coli FJP10 harboring vector, FtsY-NG, or FtsY-NG + 1 were analyzed by Western blotting with anti-FtsY antibodies (9).
