Fig. 4.

Hh response regulates stromal composition during cerulein-enhanced oncogenesis. (A–I) Confocal images of pancreata of cerulein-treated KICG mice are shown. (A and B) Vismodegib decreased the expression of αSMA, an established marker for desmoplasia in PDA. (C) Conversely, SAG21k increased αSMA expression. (D–F) Expression of Col I, another marker for desmoplasia, was decreased by vismodegib and increased by SAG21k. (G and H) Pancreata from vehicle- and vismodegib-treated mice exhibit cells expressing CD45, suggestive of hematopoietic origin, dispersed in stromal areas between PanIN lesions. (I) In contrast, CD45⁺ cells are concentrated around globular PanIN structures in SAG21k-treated mice. In all drug-treatment conditions shown, there is no overlap between Gli1⁺ and CD45⁺ cells.