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. 2014 Aug 4;206(3):377–384. doi: 10.1083/jcb.201312067

Figure 2.

Figure 2.

ClpGM6 localization to the FAZ and RNAi phenotype. (A) Phase image of an uninduced T. brucei cell overlaid with the signal of IF staining of the anti-ClpGM6 antibody (red). (B) Western blot of ClpGM6 in T. brucei cells after RNAi induction. Whole-cell lysates of uninduced (0 h) and ClpGM6 RNAi-induced (24–96 h) cells were probed with the anti-ClpGM6 antibody and L8C4, which recognizes a flagellar protein PFR2 and serves as a loading control. (C) Phase image of a 96-h ClpGM6 RNAi-induced cell overlaid with the signal of IF staining of the anti-ClpGM6 antibody (red). (D) Phase image of uninduced cells with trypomastigote morphology overlaid with a fluorescence image of DAPI, which stains DNA. (E) Phase image of 48-h ClpGM6 RNAi-induced cells showing epimastigote-like morphology overlaid with a fluorescence image of DAPI. (F) SEM image of an uninduced trypomastigote. (G) SEM of a ClpGM6 RNAi-induced epimastigote-like cell, in which a large part of the flagellum is not attached to the cell body. (H) SEM of a ClpGM6 RNAi-induced cell, with a flagellum emerging on the cell surface close to the anterior end of the cell. Bars: (A and C) 4 µm; (D and E) 10 µm; (F, applies to G and H) 2 µm.