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. 2014 Jul 13;2014:187105. doi: 10.1155/2014/187105

Figure 2.

Figure 2

The miRNA expression levels from deep sequencing and validation with qRT-PCR. (a) The scatter plot shows the expression levels of known miRNAs in SW480/siRNA-control and SW480/STAT3-siRNA cells. Blue dots: equally expressed miRNAs between SW480/siRNA-control and SW480/STAT3-siRNA. Red dots: miRNAs in SW480/STAT3-siRNA are upexpressed compared to SW480/siRNA-control (adjusted P < 0.05). Green dots: miRNAs in SW480/STAT3-siRNA are downexpressed compared to SW480/siRNA-control (adjusted P < 0.05). (b) The validation of selected up- and downexpressed known miRNAs indicates a strong correlation (Pearson's correlation = 0.97) between deep sequencing data and the qRT-PCR results.