Table 2.

Primers used for SYBR Green PCR based amplification of TLRs in this study.

Toll receptors	Primers	Sequence	NCBI accession reference number	Start site	Stop site	Product size (bp)
TLR 3	Forward	5′-GTG CCA GAA ACT TCC CAT GT-3′	NM_003265.2	383	402	231
	Reverse	5′-TCC AGC TGA ACC TGA GTT CC-3′		594	613
TLR 7	Forward	5′-AAT GTC ACA GCC GTC CCT AC-3′	NM_016562.3	782	801	223
	Reverse	5′-GCG CAT CAA AAG CAT TTA CA-3′		985	1004
TLR 8	Forward	5′-TGT GAT GGT GGT GCT TCA AT-3′	XM_005274543.2	1028	1047	187
	Reverse	5′-ATG CCC CAG AGG CTA TTT CT-3′		1214	1195

Binding site refers to the site where polymerase chain reaction (PCR) primer binds to prime duplication of a complement to the existing complementary DNA (cDNA) sequence of the mRNA; bp: base pair.