Figure 1.

Representative images of equine CL immunostained for the presence of LEP in early CL (a), LEPR in mid-CL (b), GHR in mid-CL (c), Ghr-R1A in late CL ((d) characteristic increased number of vacuole in LLC and connective tissue for late CL-green triangle). Negative control with substitution of primary antibody by rabbit IgG (e) and by PBS (f). Black arrow indicates LLC, white arrow indicates SLC, and yellow arrow indicates endothelial cells. Since all cytokines/receptors stained equally throughout the estrous cycle, pictures from each luteal phase were randomly assigned.