(A) Immunohistochemical staining of MBP and CUX1 in coronal sections of wild type adult neocortex. High magnifications of boxed areas show reduced levels of myelin in layer II/III (brackets). (B) PMAS lengths (arrowheads) in Golgi-Cox-labeled adult wild type cortex. Top, representative neurons in layer II/III and layer V. Bottom, scatter plot of PMAS length versus distance of neuronal cell bodies from the pia (n=72, R2= 0.61093). (C) 3D renderings of three representative neurons. The green and blue neurons were located in layer III–IV and the red neuron was in layer V. Myelin is shown in white. Ctx, cortex; Hip, hippocampus, Th, thalamus. Scale bars, 500 μm (A, low magnification), 100 μm (A, insets), 20 μm (B), 25 μm (C).