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. 2014 Aug 5;8:74. doi: 10.3389/fnana.2014.00074

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Copyright © 2014 Koskinen and Hotulainen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PAGFP fluorescence decay standard deviation. (A) A time-lapse series of a DIV14 rat hippocampal neuron transfected with PAGFP-actin and mCherry. The numbers represent time after photo-activation. The image is pseudocolored according to the scale on the left. The outlines of the cell were derived from mCherry fluorescence. Scale bar, 1 μm. (B) The PAGFP-actin fluorescence decay data from DIV14 hippocampal neuron spines. The gray traces represent individual spines and the red mean ± SD, n = 5 spines. (C) The standard deviation of the mean PAGFP fluorescence decay curve from (B). The red line represents the average of 2.2%.