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. 2014 Aug 5;5:276. doi: 10.3389/fphys.2014.00276

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Copyright © 2014 Moraes, Diaz-Albiter, Faria, Sant'Anna, Dillon and Genta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative expression of GHF18 members in different stages of development of Lutzomyia longipalpis as determined by Multiplex RT-PCR (Ribosomal protein 60 used as constitutive gene). Chitinases amplified were: (A) LlChit2; (B) LlChit3; (C) LlChit4; (D) LlChit5, and (E) LlIDFG. The experimental groups used in the experiments were larvae (L4), pupae (P), unfed male adults (UM), sugar-fed male adults (SM), unfed female adults (UF), sugar-fed female adults (SF), and blood-fed female adults (BF). Bars represent the mean ± SE from 3 independent experiments. The statistics were done using ANOVA with Turkey post-test and student's t-test. Asterisks indicate statistically significant differences in the samples.