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. 2010 Sep 8;30(36):11983–11993. doi: 10.1523/JNEUROSCI.1029-10.2010

Figure 4.

Figure 4.

PYK2 kinase activity is required for LTD. A, Overexpression of K457A-PYK2 inhibits AMPAR transmission. WT-PYK2 overexpression data from Figure 3C is repeated here for comparison. EPSC amplitude is shown in picoamperes. K457A PYK2 paired recordings: n = 13 pairs; p = 0.036 versus untransfected control, paired Student's t test. Calibration: 20 pA/100 ms. B, Both WT-PYK2* and K457A-PYK2*, in the presence of PYK2 shRNA, depress baseline AMPAR transmission. EPSC amplitude is shown in picoamperes. PYK2 shRNA and shRNA-resistant PYK2 constructs were mixed 1:1 for transfections. WT-PYK2* + shRNA paired recordings: n = 13 pairs; p = 0.036 versus untransfected control, paired Student's t test. K457A-PYK2* + shRNA paired recordings: n = 12 pairs; p = 0.026 versus untransfected control, paired Student's t test. Calibration: 20 pA/100 ms. C, D, WT-PYK2* fully restores LTD (C), whereas K457A-PYK2* only partially rescues LTD (D), in the presence of PYK2 shRNA. WT-PYK2* + shRNA: n = 10 pairs; p = 0.91, paired Student's t test. K457A-PYK2* + shRNA: n = 11 pairs; p = 0.031, paired Student's t test. Calibration: 50 pA/25 ms.