A. Percent reactivation of latently infected J-lat cells 24 h after treatment with either TNF alone (solid black bar, upper) or Prostratin alone (solid black bar, lower), or one of the 85 noise enhancers alone (purple bars), or in combination with TNF (red, upper) or Prostratin (red, lower). Synergy (Mean Bliss Score) is calculated relative to TNF-only or Prostratin-only control. The majority of noise enhancers synergize with TNF and Prostratin to enhance latent reactivation. Black arrows identify noise enhancers analyzed in Fig. 4B. The asterisk indicates noise enhancers that increase reactivation but without significant synergy. B. Correlation between a compound’s noise enhancement (from Fig. 2B) and its reactivation synergy with TNF (from panel A). Each data point represents a moving average of 10 compounds. C.
Left: Representative flow cytometry histogram for reactivation with TNF + noise enhancer cocktail (red) compared to TNF alone (blue). Noise enhancers amplify mean expression when used in conjunction with an activator (black arrow). Right: For all the noise enhancer + TNF combinations that synergized, the mean GFP positively correlates with reactivation %.