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. Author manuscript; available in PMC: 2015 Jun 26.
Published in final edited form as: Cell Rep. 2014 Jun 5;7(6):1926–1939. doi: 10.1016/j.celrep.2014.05.021

Figure 2. Mechano-sensing apparatuses function independently of age to generate actin stress fibers, focal adhesions, and ERK activation.

Figure 2

(A) Representative immunofluorescence of F-actin (green) in cKit+ HMEC from a young strain (240L, 19y) and an older strain (122L, 66y) on PA gels of increasing stiffness. (B and C) Image quantification of F-actin homogeneity using feature detection (B, p<0.0001 and r2=0.8297, slope= −6,805e-5 ± 9,749e-6, n=3; C, p<0.001 and r2=0.6853, slope=-8,454e-5 ± 1,811e-5, n=3). The slopes are not significantly different (p=0.5699). Representative immunofluorescence images of pFAK (red) and vinculin (green), which overlapped (yellow), from confocal microscopy are shown in cKit+ HMEC from (D) a young strain (240L 19y) and (E) an older strain (122L 66y). Cells are shown at the substrata interface. Bars represent 20µm. (F and G) Image quantification of vinculin and pFAK homogeneity (F, p<0.001 and r2=0.7456, slope=-6,512e-5 ± 1,203e-5, n=3; G, p<0.001 and r2=0.7581, slope=-6,356e-5 ± 1,136e-5, n=3). The slopes are not significantly different (p=0.8124). Data are means ± s.e.m. (H and I) Ratio of pERK positive to ERK positive cell number in cKit+ HMEC from <30y (n=3) and >55y (n=3) strains on 200Pa and 2350Pa.