Table 1.
Immunohistochemical markers used in this study
| Name | Company | Type | Antibody dilution | Function | Reactivity |
|---|---|---|---|---|---|
| Cytokeratin AE1/AE3* | BioGenex Laboratories, San Ramon, California, USA. Catalogue # AM0751-5M | Mouse monoclonal | Prediluted antibody | Recognizes high- and low-molecular-weight cytokeratins | Bile duct epithelium, BPDECs and oval cells |
| Muscle Specific actin (MSA) | BioGenex Laboratories, Catalogue # MU090-UC | Mouse monoclonal (HHF35), IgG1 | 1 in 100 | Reacts with 42 kD protein specific for actin in skeletal, cardiac, and smooth muscle | Periductal smooth muscle cells, activated stellate cells |
| Proliferating Cell Nuclear Antigen (PCNA) | BioGenex Laboratories, Catalogue # MU252-UC | Mouse monoclonal (PC10), IgG2a | 1 in 250 | Reacts with a 36 kD non-histone nuclear protein auxiliary of DNA polymerrases δ and ε enzymes necessary for DNA synthesis | Cell in the G1, S and G2/Mphases of cell cycle |
| Red fluorescent protein | Abcam, Cambridge, Massachusetts , USA. Catalogue # ab34771 | Rabbit polyclonal | 1 in 500 | Reacts with RFP protein full length amino acid sequence (234aa) from the mushroom polyp coral Discosoma | Transgenic hepatocytes expressing the RFP |
*
AE1 recognizes CK 10, 14, 15, 16 and 19; AE3 recognizes CK 1, 2, 3, 4, 5, 6 and 8