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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Biogerontology. 2014 Jun 26;15(4):347–366. doi: 10.1007/s10522-014-9506-3

Fig. 5. Role of sirtuins and other HDACs in regulation of WRN acetylation.

Fig. 5

a) 8-D cells were incubated with or without 1 mM MMS, 5 mM nicotinamide (Nico), and/or 10 uM TSA for 4 h before harvest for IP with anti-acetylated lysine antibody. IP products (upper panel) and cell lysates (50 ug each, lower panel) were subjected to Western blotting with anti-WRN antibody. c) 8-D cells were incubated with or without 2 mM HU, 5 mM Nico, and/or 10 uM TSA for 10 h before harvest for IP using anti-acetylated lysine antibody. IP products (upper panel) and cell lysates (lower panel, 30 ug each) were analyzed by Western blotting with anti-WRN antibody. e) 8-D cells were incubated with 5 mM Nico and/or 10 uM TSA for 4 h. Cell lysates were processed for IP (upper panel) and direct analysis (lower panel) as described in A. b, d, f) Bar graphs for WRN acetylation for experiments performed in A, C, and E, respectively (mean ± SEM of 3 independent experiments; * = p < 0.05 when compared with untreated cells, and # = p < 0.05 when compared with MMS (in B) or HU (in D) alone