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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Gene Ther. 2014 Mar 27;21(5):507–513. doi: 10.1038/gt.2014.24

Figure 1. PRELP Inhibits Normal Human Serum Mediated Cell Lysis.

Figure 1

(A) Schematic of pAAV2-PRELP containing the human PRELP cDNA regulated by the CAG promoter and negative control plasmid pAAV2-pA. (B) Western blot analyses of media/lysate from human ARPE-19 cells transfected with pAAV2-PRELP or pAAV2-pA, indicating a band of approximately 48 kDa associated with human PRELP specifically in media/lysate of pAAV2-PRELP transfected ARPE-19 cells. (C) Representative FACS-plots indicating the number of Hepa-1c1c7 cells taking up propidium iodide following incubation in 1.5% normal human serum (NHS) preconditioned with media from human ARPE-19 cells transfected with pAAV2-PRELP or pAAV2-pA. (D) Quantification of FACS-lysis assays. PRELP reduced cell lysis by 18.74±3.95% (mean value±SEM; **p=0.0015; n=5). CAG: chicken β-actin promoter with CMV enhancer; LITR/RITR: left/right AAV inverted terminal repeat; pA: poly-adenylation sequence.