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. 2014 Aug 5;9(8):e103784. doi: 10.1371/journal.pone.0103784

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid Genotype and/or relevant characteristics Reference or source
P. aeruginosa
PAO1 (ATCC15692) Prototroph American type culture collection
PA14 Prototroph [20]
TR1 Prototroph; cystic fibrosis isolate [21]
PAO1 araCPBAD tolB PAO1 with an arabinose-inducible additional copy of tolB inserted into the attB neutral site This work
PAO1 ΔtolB araCPBAD tolB PAO1 araCPBAD tolB deleted of the endogenous copy of tolB This work
PA14 araCPBAD tolB PA14 with an arabinose-inducible additional copy of tolB inserted into the attB neutral site This work
PA14 ΔtolB araCPBAD tolB PA14 araCPBAD tolB deleted of the endogenous copy of tolB This work
TR1 araCPBAD tolB TR1 with an arabinose-inducible additional copy of tolB inserted into the attB neutral site This work
TR1 ΔtolB araCPBAD tolB TR1 araCPBAD tolB deleted of the endogenous copy of tolB This work
E. coli
S17.1λpir thi pro hsdR hsdM+ recA RP4-2-Tc::Mu-Km::Tn7 λpir, GmR [22]
DH5αF' recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169[φ80 dlacZΔM15], NalR [23]
Plasmid
pBluescript-II KS+ Cloning vector; ColE1 replicon; ApR Stratagene
pDM4 Suicide vector; sacBR, oriR6K; CmR [23]
pDM4ΔtolB pDM4 derivative for tolB in-frame deletion; CmR This work
pBEM9 Vector carrying the araCPBAD regulatory region with an altered RBS for stringent arabinose-dependent control in P. aeruginosa; ApR [24]
pBEM9-tolB pBEM9 derivative carrying the tolB coding sequence cloned by HindIII/EcoRI digestion downstream of the araCPBAD regulatory region This work
mini-CTX1 Self-proficient integration vector with tet, Ω-FRT-attP-MCS, ori, int, and oriT; TcR [26]
mini-CTX1-araCPBAD tolB mini-CTX1 derivative carrying the araCPBAD tolB from pBEM9 cloned by XhoI/EcoRI digestion This work