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. 2014 Aug 5;9(8):e103954. doi: 10.1371/journal.pone.0103954

Figure 5. Level of DNA methylation in NOR-1 and NOR-2 of Q.robur.

Figure 5

A. Immunofluorescence using an antibody against 5-mC coupled with FISH using the 18S rDNA probe revealed the presence of 5-mC in both 45S rDNA loci. B. Co-localization analysis of 5-mC and FISH signals showed stronger co-localization in minor rDNA sites (NOR-2) when compared to major rDNA sites (NOR-1); N = 26. Number of asterisks indicates p<0.01; error bars represent the standard error of the mean. Letter “n” marks the nucleoli. Scale bar is 5 µm.