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. 2014 Aug 5;9(8):e103954. doi: 10.1371/journal.pone.0103954

Figure 10. RT-PCR analysis of 18S rRNA transcription.

Figure 10

RT-PCR analysis detected an increased level of 18S rRNA transcripts after treatment of root tips with 5-aza-2′-dC. C – control, T – treatment with 5-aza-2′-dC. OD/AU is optical density (in arbitrary units) of RT-PCR product peaks after electrophoresis in agarose gel and staining with ethidium bromide. The 18S rRNA (1830 bp band) transcription increased more than twofold after treatment with 5-aza-2′-dC, while the transcription level of the Actin3 gene (655 bp, used as control) remained essentially at the same level.