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. 2014 Aug 5;9(8):e104020. doi: 10.1371/journal.pone.0104020

Figure 4. TLR4 inhibition increases endothelial modulation of vasoconstrictor responses.

Figure 4

Concentration-response curves to phenylephrine in intact (E+) and endothelium denuded (E−) aortic segments from (A) Wistar and (B) SHRs treated with a non-specific IgG (1 µg/day, 15 days) and (C) SHRs treated with anti-TLR4 antibody (1 µg/day, 15 days). (D) Differences in the area under the concentration-response curve (dAUC) in the E– and E+ segments; AUC was calculated from the individual curve plots; the differences are expressed as a percentage of the AUC for the corresponding control situation. (E) Contractile response to L-NAME (100 µM) after phenylephrine (1 µM) contraction in segments from Wistar and SHRs treated with a non-specific IgG and SHRs treated with anti-TLR4 antibody; the results are expressed as the percentage of the previous contraction induced by phenylephrine. The results are the mean±SEM. *P<0.05 vs. E+ or vs. Wistar, #P<0.05 vs. SHR using two-way ANOVA and Bonferroni post-test. The number of animals used is shown in parentheses.