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. 2014 Aug 5;9(8):e104338. doi: 10.1371/journal.pone.0104338

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© 2014 Chakraborty et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunoblots of RyR2, pRyR2-S2809 and pRyR2-S2815 of heart homogenates from 10-day old Ryr2^+/+/AC3-C (WC) and/AC3-I (WI) and Ryr2^ADA/ADA/AC3-C (HC) and/AC3-I (HI) mice. Equal amounts of proteins (20 µg) were loaded on gels. (B) Intensity of RyR2 protein bands normalized for Ryr2^+/+/AC3-C protein band intensities. (C and D) pRyR2/RyR2 on S2809 and S2815, respectively. Data were obtained analyzing proteins from 4–6 hearts of each genotype and are the mean ± SEM of 13 (B), 4 (C) and 5–7 (D) determinations using two way ANOVA. *p<0.05 compared with WC and WI. ^#p<0.05 compared with HC in blot of total RyR2 shown in B.